Regenerable bead-based microfluidic device with integrated thin-film photodiodes for real-time monitoring of DNA detection

Recently, systems for rapid diagnosis of diseases and infections have motivated the development new DNA detection platforms. However, these platforms failed to reach market mainly because potential risk misdiagnosis. Thus, developing a system with an effective internal control, guaranteeing veracity result, could help overcome this barrier. The possibility regenerate microfluidic biosensing would allow implementation aforementioned control system's reusability, resulting in cost reduction. Nanoporous microbead-based coupled on-chip signal transduction can potentially significant enhancement sensitivity applications. In paper, we report regenerable bead-based device that employs integrated thin-film photodiodes real-time monitoring molecular recognition. identification target complementary is accomplished, followed by regeneration chip using 0.3 M NaOH non-complementary DNA. best condition immobilization probe was studied, limits 1.2 nM 3.1 were obtained buffer human plasma, respectively, detected fluorescence assays. Moreover, chemiluminescence strategy provided highest detect limit 78.5 pM. High-sensitivity assay cycles be performed without loss DNA, demonstrating portability, reproducibility. • A developed. Regeneration accomplished flowing through beads. Up four density. p-i-n optical readout. Target pM range assays, amplification.